Volume 2 (Issue 3)

pp. 100-104

Open Access

Research paper

Analysis of Isolated Yeast Strains from Different Sources for Bio-ethanol Production

Sunil Kumar Verma, Virendra Singh, Devendra Singh*

SKV, DS: Department of Biotechnology, BN College of Engineering and Technology, Lucknow 226201, Uttar Pradesh, India

VS: Department of Pharmacy, FS University, Shikohabad 283135, Uttar Pradesh, India


*Corresponding author: Devendra Singh; Email: dev7600@gmail.com

DOI:

Received: 

07 August 2022

Published:

10 September 2022

Cite as: Verma, S. K., Singh, V., & Singh, D. (2022). Analysis of Isolated Yeast Strains from Different Sources for Bio-ethanol Production. Inventum Biologicum, 2(3), 100–104. https://doi.org/10.5281/zenodo.7067346

Abstract

The generation of bioethanol and alcoholic beverages uses yeast (Saccharomyces cerevisiae) on a worldwide scale; however, its activity is hampered by the buildup of intracellularly produced ethanol on cell viability. In the past, various yeast strains and their condition of culture is being employed to lessen the ethanol stress impact on the expression of the gene; however, these methods are affected by a range of factors. But there are similarities observed in the gene ontology because of the effects of ethanol, which indicates that the energy production constraints compromise Saccharomyces cerevisiae's ability to respond to stress from ethanol by increasing the expression of genes related to glycolysis and the mitochondrial TCA cycle while decreasing the expression of ATP-mediated growth-associated processes. Saccharomyces cerevisiae was used as the reference strain in the current study. Different strains of yeast were isolated from different materials, and the growth of the strains isolated was monitored.
Further, the ethanol measurement was carried out using the DNS method. The yeast strain with the best growth rate was used in the alcohol tolerance test to withstand ethanol stress. In the future, the molecular underpinnings of the yeast strain's tolerance to alcohol can help with genetic engineering to create methods for enhancing its function under ethanol stress.

Keywords:

Yeast, Bio-ethanol, Alcohol tolerance, Growth kinetics, DNS test

References

  1. Cabañas, K. T., Peña-Moreno, I. C., Parente, D. C., García, A. B., Gutiérrez, R. G., & de Morais Jr, M. A. (2019). Selection of Saccharomyces cerevisiae isolates for ethanol production in the presence of inhibitors. 3 Biotech, 9(1), 6. https://doi.org/10.1007/s13205-018-1541-3

  2. Della-Bianca, B. E., de Hulster, E., Pronk, J. T., van Maris, A. J. A., & Gombert, A. K. (2014). Physiology of the fuel ethanol strain Saccharomyces cerevisiae PE-2 at low pH indicates a context-dependent performance relevant for industrial applications. FEMS Yeast Research, 14(8), 1196–1205. https://doi.org/10.1111/1567-1364.12217

  3. Demirbas, A. (2008). Biofuels sources, biofuel policy, biofuel economy and global biofuel projections. Energy Conversion and Management, 49(8), 2106–2116. https://doi.org/10.1016/j.enconman.2008.02.020

  4. Gnansounou, E., & Dauriat, A. (2005). Ethanol fuel from biomass: A review. Journal of Scientific and Industrial Research, 64, 809–821.

  5. Kumar, R. S., Shankar, T., & Anandapandian, K. T. K. (2011). Characterization of alcohol-resistant yeast Saccharomyces cerevisiae isolated from Toddy. Int. Research Journal of Microbiology, 2(10), 399–405.

  6. Nagodawithana, T. W., & Steinkraus, K. H. (1976). Influence of the rate of ethanol production and accumulation on the viability of Saccharomyces cerevisiae in “rapid fermentation” Applied and Environmental Microbiology, 31(2), 158–162. https://doi.org/10.1128/aem.31.2.158-162.1976

  7. Nordin, M. A., Razak, F. A., & Himratul-Aznita, W. H. (2015). Assessment of antifungal activity of bakuchiol on oral-associated Candida spp. Evidence-Based Complementary and Alternative Medicine: eCAM, 1, 1–7.

  8. Parapouli, M., Vasileiadis, A., Afendra, A. S., & Hatziloukas, E. (2020). Saccharomyces cerevisiae and its industrial applications. AIMS Microbiology, 6(1), 1–31. https://doi.org/10.3934/microbiol.2020001

  9. Ramos, C. L., Duarte, W. F., Freire, A. L., Dias, D. R., Eleutherio, E. C. A., & Schwan, R. F. (2013). Evaluation of stress tolerance and fermentative behavior of indigenous Saccharomyces cerevisiae. Brazilian Journal of Microbiology, 44(3), 935–944. https://doi.org/10.1590/S1517-83822013005000051

  10. Schmer, M. R., Vogel, K. P., Varvel, G. E., Follett, R. F., Mitchell, R. B., & Jin, V. L. (2014). Energy potential and greenhouse gas emissions from bioenergy cropping systems on marginally productive cropland. PLOS ONE, 9(3), e89501. https://doi.org/10.1371/journal.pone.0089501

  11. Sivasakthivelan, P., Saranraj, P., & Sivasakthi, S. (2014). Production of ethanol by Zymomonas mobilis and Saccharomyces cerevisiae using sunflower head wastes – A comparative study. International Journal of Microbiology Research, 5(3), 208–216.

  12. Stanley, D., Bandara, A., Fraser, S., Chambers, P. J., & Stanley, G. A. (2010). The ethanol stress response and ethanol tolerance of Saccharomyces cerevisiae. Journal of Applied Microbiology, 109(1), 13–24. https://doi.org/10.1111/j.1365-2672.2009.04657.x

  13. Tahir, A., Aftab, M., & Farasat, T. (2010). Effect of cultural conditions on ethanol production by locally isolated Saccharomyces cerevisiae BIO-07. Journal of Applied Pharmacy, 3(2), 72–78.

  14. Tenkolu, G. A., Kuffi, K. D., & Gindaba, G. T. (2022). Optimization of fermentation condition in bioethanol production from waste potato and product characterization. Biomass Conversion and Biorefinery, 1-19. https://doi.org/10.1007/s13399-022-02974-4

  15. Watanabe, D., Wu, H., Noguchi, C., Zhou, Y., Akao, T., & Shimoi, H. (2011). Enhancement of the Initial Rate of ethanol Fermentation Due to dysfunction of Yeast Stress Response Components Msn2p and/or Msn4p. Applied and Environmental Microbiology, 77(3), 934–941. https://doi.org/10.1128/AEM.01869-10

  16. Yamaoka, C., Kurita, O., & Kubo, T. (2014). Improved ethanol tolerance of Saccharomyces cerevisiae in mixed cultures with Kluyveromyces lactis on high-sugar fermentation. Microbiological Research, 169(12), 907–914. https://doi.org/10.1016/j.micres.2014.04.007

Funding Information

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

Declaration of Conflict

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.